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These results suggest that loss-of-function mutations in the enriched targets and gain-of-function mutations in the depleted targets cause clonal expansion in normal esophagus

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For example, in ketotic dairy cows, the ruminal level of the tryptophan metabolite indole-3-acetic acid (IAA) is significantly reduced and is accompanied by downregulation of epithelial tight junction proteins, increased inflammatory mediators, and impaired barrier function